Hotspot

rpoA-rps11

LSC · 75894-75957 · 64 bp

Back to Danthoniopsis

Pi 0.0312

MarkerSeek score 70.50

Primer available yes

Label rpoA-rps11

Feature Metadata

label_name: rpoA-rps11
feature_type: igs
region: LSC
length_bp: 64
variable_sites: 2
pi: 0.0312
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA-rps11_p1	GTGTTTGGGTGAGTCCTGTT	GATGTAACACCTATGCCGCA	50.0	50.0	57.9	58.1	158 bp	1.0	80.2
rpoA-rps11_p2	GTGTTTGGGTGAGTCCTGTT	TGATGTAACACCTATGCCGC	50.0	50.0	57.9	58.1	159 bp	1.0	80.2
rpoA-rps11_p3	GGTGAGTCCTGTTACCTCCT	GATGTAACACCTATGCCGCA	55.0	50.0	58.1	58.1	151 bp	1.0	80.2
rpoA-rps11_p4	GGTGAGTCCTGTTACCTCCT	TGATGTAACACCTATGCCGC	55.0	50.0	58.1	58.1	152 bp	1.0	80.2
rpoA-rps11_p5	GTGTTTGGGTGAGTCCTGTT	ACACCTATGCCGCATAATGG	50.0	50.0	57.9	58.1	152 bp	1.0	79.8