Hotspot

psaJ-rpl33

LSC · 65263-65785 · 523 bp

Back to Danthoniopsis

Pi 0.00956

MarkerSeek score 62.60

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 523
variable_sites: 5
pi: 0.00956
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TTTGGGGCTTTAGCAGGTTT	ACCCTTTCGAACACAACTGA	45.0	45.0	57.9	57.3	670 bp	1.0	74.2
psaJ-rpl33_p2	TTTGGGGCTTTAGCAGGTTT	TGGTACCCTTTCGAACACAA	45.0	45.0	57.9	57.0	674 bp	1.0	71.8
psaJ-rpl33_p3	TTTGGGGCTTTAGCAGGTTT	TTGGTACCCTTTCGAACACA	45.0	45.0	57.9	57.0	675 bp	1.0	71.8
psaJ-rpl33_p4	TTTGGGGCTTTAGCAGGTTT	CCCCGTCAATTCCTTATTGGT	45.0	47.6	57.9	58.0	691 bp	1.0	71.7
psaJ-rpl33_p5	ATGGTTTGGGGCTTTAGCAG	ACCCTTTCGAACACAACTGA	50.0	45.0	58.4	57.3	674 bp	1.0	71.5