Hotspot

petA-psbJ

LSC · 61004-61931 · 928 bp

Back to Danthoniopsis

Pi 0.0194

MarkerSeek score 55.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 928
variable_sites: 18
pi: 0.0194
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GCAATCCTAATGTGGGAGGG	TGCGGGTTGGTTATTTGTCA	55.0	45.0	58.0	58.0	1774-1775 bp	1.0	80.7
petA-psbJ_p2	GCAATCCTAATGTGGGAGGG	TCCCTATTCATTGCGGGTTG	55.0	50.0	58.0	57.9	1785-1786 bp	1.0	80.5
petA-psbJ_p3	GCAATCCTAATGTGGGAGGG	CACGGATTAGCTGTACCCAC	55.0	55.0	58.0	58.1	1556-1557 bp	1.0	80.5
petA-psbJ_p4	CTTGTGATAGGCCCTGTTCC	TGCGGGTTGGTTATTTGTCA	55.0	45.0	58.0	58.0	2123-2124 bp	1.0	80.4
petA-psbJ_p5	TATTATACCTCCCGGGCCAG	TGCGGGTTGGTTATTTGTCA	55.0	45.0	58.1	58.0	1845-1846 bp	1.0	80.4