Hotspot

ndhC-trnV-UAC

LSC · 50704-51606 · 903 bp

Back to Danthoniopsis

Pi 0.0178
MarkerSeek score 60.40
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
903
variable_sites
16
pi
0.0178
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CCAAAATAGGAATAAGGCCTGC GGTTCGAATCCGTATAGCCC 45.5 55.0 57.4 57.9 995-998 bp 1.0 60.1
ndhC-trnV-UAC_p2 CCAAAATAGGAATAAGGCCTGC AGCCCAATGCAGAAAAATGC 45.5 45.0 57.4 57.9 1088-1091 bp 1.0 59.8
ndhC-trnV-UAC_p3 CCAAAATAGGAATAAGGCCTGC CGGTTCGAATCCGTATAGCC 45.5 55.0 57.4 58.3 996-999 bp 1.0 58.3
ndhC-trnV-UAC_p4 CCAAAATAGGAATAAGGCCTGC CGAGAAGGTCTACGGTTCGA 45.5 55.0 57.4 58.9 1008-1011 bp 1.0 53.4
ndhC-trnV-UAC_p5 CCAAAATAGGAATAAGGCCTGC AAGGTCTACGGTTCGAATCC 45.5 50.0 57.4 57.0 1004-1007 bp 1.0 53.1