Hotspot

trnC-GCA-rpoB

LSC · 18842-19884 · 1043 bp

Back to Danthoniopsis

Pi 0.0096

MarkerSeek score 61.00

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 1043
variable_sites: 10
pi: 0.0096
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	AAAGGATTTGCAGTCCCCTG	AAGTTCTTCTGCCAAGCCTT	50.0	45.0	58.1	57.6	1190-1191 bp	1.0	77.2
trnC-GCA-rpoB_p2	AAAGGATTTGCAGTCCCCTG	AGTTCTTCTGCCAAGCCTTG	50.0	50.0	58.1	58.4	1189-1190 bp	1.0	77.1
trnC-GCA-rpoB_p3	AAAGGATTTGCAGTCCCCTG	CAAGTTCTTCTGCCAAGCCT	50.0	50.0	58.1	58.4	1191-1192 bp	1.0	77.1
trnC-GCA-rpoB_p4	AAAGGATTTGCAGTCCCCTG	GTTCTTCTGCCAAGCCTTGA	50.0	50.0	58.1	58.4	1188-1189 bp	1.0	77.0
trnC-GCA-rpoB_p5	AAAGGATTTGCAGTCCCCTG	TCAAGTTCTTCTGCCAAGCC	50.0	50.0	58.1	58.4	1192-1193 bp	1.0	77.0