Hotspot

psbI-trnS-GCU

LSC · 7893-8014 · 122 bp

Back to Danthoniopsis

Pi 0.0417

MarkerSeek score 65.00

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 122
variable_sites: 5
pi: 0.0417
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	TAATCCTGGGCGTGAGGAG	TAAAGCGGCGGATTGCTAAT	57.9	45.0	58.5	58.0	210-212 bp	1.0	70.5
psbI-trnS-GCU_p2	GTAATCCTGGGCGTGAGGA	TAAAGCGGCGGATTGCTAAT	57.9	45.0	58.8	58.0	211-213 bp	1.0	68.0
psbI-trnS-GCU_p3	TAATCCTGGGCGTGAGGAG	ATGGCTGAGTGGACTAAAGC	57.9	50.0	58.5	57.6	224-226 bp	1.0	67.3
psbI-trnS-GCU_p4	GTAATCCTGGGCGTGAGGAG	TAAAGCGGCGGATTGCTAAT	60.0	45.0	59.9	58.0	211-213 bp	1.0	67.2
psbI-trnS-GCU_p5	TAATCCTGGGCGTGAGGAG	GAGAGATGGCTGAGTGGACT	57.9	55.0	58.5	58.5	229-231 bp	1.0	66.4