Hotspot

rps16-trnQ-UUG

LSC · 5503-6765 · 1263 bp

Back to Danthoniopsis

Pi 0.0143
MarkerSeek score 54.90
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1263
variable_sites
18
pi
0.0143
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1337-1367 bp 1.0 79.5
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA CTCGGAGGTTCGAATCCTTC 45.0 55.0 58.1 57.8 1341-1371 bp 1.0 78.4
rps16-trnQ-UUG_p3 TGATCGTGTCCTTCAAGTCG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.7 58.1 1351-1381 bp 1.0 77.8
rps16-trnQ-UUG_p4 TGATCGTGTCCTTCAAGTCG CTCGGAGGTTCGAATCCTTC 50.0 55.0 57.7 57.8 1355-1385 bp 1.0 76.7
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA TACTCGGAGGTTCGAATCCT 45.0 50.0 58.1 57.6 1343-1373 bp 1.0 76.6