Hotspot

petB

LSC · 70535-71980 · 1446 bp

Back to Danthonia

Pi 0.00277

MarkerSeek score 60.70

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1446
variable_sites: 6
pi: 0.00277
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	GGTAGTTCGACCGTGGATTT	ACCACAACCTACCCGATATGA	50.0	47.6	57.6	58.2	1544-1566 bp	1.0	67.4
petB_p2	GGTAGTTCGACCGTGGATTT	ACCACAACCTACCCGATATG	50.0	50.0	57.6	56.7	1544-1566 bp	1.0	67.1
petB_p3	GGTAGTTCGACCGTGGATTT	TGAAATACCACAACCTACCCG	50.0	47.6	57.6	57.7	1550-1572 bp	1.0	66.7
petB_p4	ATTCTGGTAGTTCGACCGTG	ACCACAACCTACCCGATATGA	50.0	47.6	57.4	58.2	1549-1571 bp	1.0	65.3
petB_p5	CATTCTGGTAGTTCGACCGT	ACCACAACCTACCCGATATGA	50.0	47.6	57.4	58.2	1550-1572 bp	1.0	65.3