Hotspot

ndhG

SSC · 108426-108956 · 531 bp

Back to Danthonia

Pi 0.00251

MarkerSeek score 34.60

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 2
pi: 0.00251
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 34.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	AAGAATTTTGACTCATTCTAAGT	CCTGAATCTTTTAGTTTTAGTCAGT	26.1	32.0	52.0	55.3	624 bp	1.0	40.3
ndhG_p2	TGAAATTCTAAGAATTTTGACTCA	CCTGAATCTTTTAGTTTTAGTCAGT	25.0	32.0	52.9	55.3	633 bp	1.0	40.3
ndhG_p3	TCTAAGAATTTTGACTCATTCTAAGT	CCTGAATCTTTTAGTTTTAGTCAGT	26.9	32.0	54.7	55.3	627 bp	1.0	40.3
ndhG_p4	AAGAATTTTGACTCATTCTAAGT	CCTGAATCTTTTAGTTTTAGTCAGTT	26.1	30.8	52.0	55.9	624 bp	1.0	40.3
ndhG_p5	AAGAATTTTGACTCATTCTAAGT	CCTGAATCTTTTAGTTTTAGTCAGTTC	26.1	33.3	52.0	56.9	624 bp	1.0	40.3