Hotspot

ndhF

SSC · 100718-102934 · 2217 bp

Back to Danthonia

Pi 0.00406

MarkerSeek score 49.00

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2217
variable_sites: 13
pi: 0.00406
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACGGAGACTTGCTTCACATT	AGGTTTGGTGAATCGGAAACA	45.0	42.9	57.4	58.1	2586 bp	1.0	67.6
ndhF_p2	ACGGAGACTTGCTTCACATT	ACAGTTGCAAAACAAGAGAGC	45.0	42.9	57.4	57.6	2728-2870 bp	1.0	64.7
ndhF_p3	ACGGAGACTTGCTTCACATT	GGTTTGGTGAATCGGAAACA	45.0	45.0	57.4	56.5	2585 bp	1.0	64.4
ndhF_p4	TCATCGGAAAGAGGTCTACGA	AGGTTTGGTGAATCGGAAACA	47.6	42.9	58.0	58.1	2543 bp	1.0	63.9
ndhF_p5	TCGGAAAGAGGTCTACGAAGA	AGGTTTGGTGAATCGGAAACA	47.6	42.9	57.9	58.1	2540 bp	1.0	63.5