Hotspot

rpl32-trnL-UAG

SSC · 104051-104364 · 314 bp

Back to Danthonia

Pi 0.0255
MarkerSeek score 63.30
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
314
variable_sites
12
pi
0.0255
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 CGAGAACCCAAAACCAAAAGG TCCTAAGAGCAGCGTGTCTA 47.6 50.0 57.9 57.9 414-1256 bp 1.0 71.6
rpl32-trnL-UAG_p2 CGAGAACCCAAAACCAAAAGG AGCAGCGTGTCTACCAATTT 47.6 45.0 57.9 57.8 407-1249 bp 1.0 71.2
rpl32-trnL-UAG_p3 CGAGAACCCAAAACCAAAAGG GCTTGAGCACTGCTTCCTAA 47.6 50.0 57.9 58.2 428-1270 bp 1.0 71.1
rpl32-trnL-UAG_p4 CGAGAACCCAAAACCAAAAGG CCTAAGAGCAGCGTGTCTAC 47.6 55.0 57.9 57.8 413-1255 bp 1.0 71.1
rpl32-trnL-UAG_p5 CGAGAACCCAAAACCAAAAGG CTAAGAGCAGCGTGTCTACC 47.6 55.0 57.9 57.8 412-1254 bp 1.0 71.1