Hotspot

ndhF-rpl32

SSC · 102935-103858 · 924 bp

Back to Danthonia

Pi 0.00844

MarkerSeek score 55.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 924
variable_sites: 11
pi: 0.00844
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGAAGTGGGAGAAGAGGGAT	TGACATCGAAGTACGTTTTT	55.0	35.0	58.1	53.2	273-1007 bp	1.0	43.8
ndhF-rpl32_p2	GGAAGTGGGAGAAGAGGGAT	TTGACATCGAAGTACGTTTT	55.0	35.0	58.1	53.2	273-1008 bp	1.0	43.8
ndhF-rpl32_p3	GGAAGTGGGAGAAGAGGGAT	TTTGACATCGAAGTACGTTT	55.0	35.0	58.1	53.2	273-1009 bp	1.0	43.8
ndhF-rpl32_p4	GGAAGTGGGAGAAGAGGGAT	TTTTGACATCGAAGTACGTT	55.0	35.0	58.1	53.2	273-1010 bp	1.0	43.8
ndhF-rpl32_p5	GGAAGTGGGAGAAGAGGGAT	TTTTTGACATCGAAGTACGT	55.0	35.0	58.1	53.2	273-1011 bp	1.0	43.8