Hotspot

rpl20-Daca_g44

LSC · 66010-66704 · 695 bp

Back to Danthonia

Pi 0.00576

MarkerSeek score 60.80

Primer available yes

Label rpl20-Daca_g44

Feature Metadata

label_name: rpl20-Daca_g44
feature_type: igs
region: LSC
length_bp: 695
variable_sites: 6
pi: 0.00576
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-Daca_g44_p1	CATTTTTGTTCGCCGTCTCC	AAATGCTAGAAAAACGCCCG	50.0	45.0	58.0	57.4	813-818 bp	1.0	75.3
rpl20-Daca_g44_p2	GTTTGAGGCAAATGAACGCA	AAATGCTAGAAAAACGCCCG	45.0	45.0	57.9	57.4	831-836 bp	1.0	74.5
rpl20-Daca_g44_p3	AGTTTGAGGCAAATGAACGC	AAATGCTAGAAAAACGCCCG	45.0	45.0	57.6	57.4	832-837 bp	1.0	72.2
rpl20-Daca_g44_p4	CATTTTTGTTCGCCGTCTCC	GTCGGGGAACATGTGCTAG	50.0	57.9	58.0	57.9	772-777 bp	1.0	71.8
rpl20-Daca_g44_p5	CATTTTTGTTCGCCGTCTCC	GGAACATGTGCTAGGGTGTA	50.0	50.0	58.0	56.9	767-772 bp	1.0	71.8