Hotspot

rpl33-rps18

LSC · 64599-64933 · 335 bp

Back to Danthonia

Pi 0.0154

MarkerSeek score 52.60

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 335
variable_sites: 7
pi: 0.0154
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 52.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	AATCGTCACAATACACCCGG	TCGAGAACGCCTACGAAAAG	50.0	50.0	58.0	58.0	537-569 bp	1.0	85.0
rpl33-rps18_p2	AATCGTCACAATACACCCGG	AACGCCTACGAAAAGGTTGT	50.0	45.0	58.0	58.0	532-564 bp	1.0	84.8
rpl33-rps18_p3	AATCGTCACAATACACCCGG	ACGCCTACGAAAAGGTTGTT	50.0	45.0	58.0	58.0	531-563 bp	1.0	84.8
rpl33-rps18_p4	AATCGTCACAATACACCCGG	TGACTGGGCTTGAAATTGCT	50.0	45.0	58.0	58.0	744-776 bp	1.0	84.3
rpl33-rps18_p5	AATCGTCACAATACACCCGG	AAAGGAATTCCCGGAGAGGA	50.0	50.0	58.0	58.0	1108 bp	0.333333	52.9