Hotspot

petA-psbJ

LSC · 59879-60788 · 910 bp

Back to Danthonia

Pi 0.00366

MarkerSeek score 61.70

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 910
variable_sites: 5
pi: 0.00366
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTCGC	TCTGGATTGGGTTCGTCTCT	50.0	50.0	57.9	58.1	1036-1037 bp	1.0	79.9
petA-psbJ_p2	CGTCCAAGGCCTTTTGTTCT	TCTGGATTGGGTTCGTCTCT	50.0	50.0	58.7	58.1	1043-1044 bp	1.0	74.8
petA-psbJ_p3	GGCCTTTTGTTCTTCTTCGC	TTCTGGATTGGGTTCGTCTC	50.0	50.0	57.9	57.2	1037-1038 bp	1.0	74.3
petA-psbJ_p4	GCCTTTTGTTCTTCTTCGCA	TCTGGATTGGGTTCGTCTCT	45.0	50.0	57.2	58.1	1035-1036 bp	1.0	74.1
petA-psbJ_p5	GGCCTTTTGTTCTTCTTCGC	TCTGGATTGGGTTCGTCTCTA	50.0	47.6	57.9	57.9	1036-1037 bp	1.0	71.6