Hotspot

rbcL-psaI

LSC · 55777-56584 · 808 bp

Back to Danthonia

Pi 0.00578

MarkerSeek score 53.90

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 808
variable_sites: 7
pi: 0.00578
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	AGCTTGCGAAGTATGGAAGG	GACATTGCAATTGCCGGAAA	50.0	45.0	58.0	57.9	940-1058 bp	1.0	79.8
rbcL-psaI_p2	ATCAAATTTGAGTTCGCGCC	GACATTGCAATTGCCGGAAA	45.0	45.0	57.7	57.9	918-1036 bp	1.0	77.9
rbcL-psaI_p3	AGCTTGCGAAGTATGGAAGG	GAAGACATTGCAATTGCCGG	50.0	50.0	58.0	58.4	943-1061 bp	1.0	77.5
rbcL-psaI_p4	TTCGCGCCGGTAGATACTAT	GACATTGCAATTGCCGGAAA	50.0	45.0	58.5	57.9	906-1024 bp	1.0	76.3
rbcL-psaI_p5	CGAAGTATGGAAGGCGATCA	GACATTGCAATTGCCGGAAA	50.0	45.0	57.5	57.9	934-1052 bp	1.0	75.9