Hotspot

trnT-UGU-trnL-UAA

LSC · 46102-46888 · 787 bp

Back to Danthonia

Pi 0.00932

MarkerSeek score 59.60

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 787
variable_sites: 11
pi: 0.00932
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	ATTTGAACCGATGACCCTCG	CCGTAGCGTCTACCGATTTC	50.0	55.0	58.0	58.2	875-900 bp	1.0	79.5
trnT-UGU-trnL-UAA_p2	CGATGACCCTCGCATTACAA	CCGTAGCGTCTACCGATTTC	50.0	55.0	58.1	58.2	867-892 bp	1.0	79.2
trnT-UGU-trnL-UAA_p3	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	845-870 bp	1.0	78.7
trnT-UGU-trnL-UAA_p4	ATTTGAACCGATGACCCTCG	CGTCTACCGATTTCGCCATA	50.0	50.0	58.0	57.6	869-894 bp	1.0	78.3
trnT-UGU-trnL-UAA_p5	CGATGACCCTCGCATTACAA	CGTCTACCGATTTCGCCATA	50.0	50.0	58.1	57.6	861-886 bp	1.0	77.9