Hotspot

petN-trnC-GCA

LSC · 18099-19014 · 916 bp

Back to Danthonia

Pi 0.00437

MarkerSeek score 61.50

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: LSC
length_bp: 916
variable_sites: 6
pi: 0.00437
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	AGCCCAAGCGAGACTTACTA	AGTTCAAATCTGGGTGCCG	50.0	52.6	58.2	58.1	965 bp	1.0	70.9
petN-trnC-GCA_p2	AGACCACCATTAAAGCAGCC	AGTTCAAATCTGGGTGCCG	50.0	52.6	58.2	58.1	981 bp	1.0	70.8
petN-trnC-GCA_p3	AGCCCAAGCGAGACTTACTA	CCAGTTCAAATCTGGGTGCC	50.0	55.0	58.2	59.1	967 bp	1.0	70.4
petN-trnC-GCA_p4	AGACCACCATTAAAGCAGCC	CCAGTTCAAATCTGGGTGCC	50.0	55.0	58.2	59.1	983 bp	1.0	70.3
petN-trnC-GCA_p5	CCACTCCTCCCCCATACTAC	AGTTCAAATCTGGGTGCCG	60.0	52.6	58.3	58.1	1021 bp	1.0	69.8