Hotspot

trnD-GUC-psbM

LSC · 16300-17193 · 894 bp

Back to Danthonia

Pi 0.00224
MarkerSeek score 60.40
Primer available yes
Label trnD-GUC-psbM

Feature Metadata

label_name
trnD-GUC-psbM
feature_type
igs
region
LSC
length_bp
894
variable_sites
3
pi
0.00224
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-psbM_p1 TTCAATTGGTCAGAGCACCG ATTAACCCTTTTGGCTGGCT 50.0 45.0 58.5 57.7 1063-1064 bp 1.0 74.0
trnD-GUC-psbM_p2 TTCAATTGGTCAGAGCACCG CCTTTTGGCTGGCTGTTTTT 50.0 45.0 58.5 57.7 1057-1058 bp 1.0 73.9
trnD-GUC-psbM_p3 TTCAATTGGTCAGAGCACCG TTAACCCTTTTGGCTGGCTG 50.0 50.0 58.5 58.7 1062-1063 bp 1.0 71.3
trnD-GUC-psbM_p4 GTTCAATTGGTCAGAGCACC ATTAACCCTTTTGGCTGGCT 50.0 45.0 57.0 57.7 1064-1065 bp 1.0 69.8
trnD-GUC-psbM_p5 GTTCAATTGGTCAGAGCACC CCTTTTGGCTGGCTGTTTTT 50.0 45.0 57.0 57.7 1058-1059 bp 1.0 69.8