Hotspot

trnG-UCC-trnM-CAU

LSC · 13579-15096 · 1518 bp

Back to Danthonia

Pi 0.00636

MarkerSeek score 56.60

Primer available yes

Label trnG-UCC-trnM-CAU

Feature Metadata

label_name: trnG-UCC-trnM-CAU
feature_type: igs
region: LSC
length_bp: 1518
variable_sites: 14
pi: 0.00636
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnM-CAU_p1	ACGAATCACACTTTTACCACT	TCTTACCGGGGCATTACTCT	38.1	50.0	55.5	57.8	1604-1652 bp	1.0	54.0
trnG-UCC-trnM-CAU_p2	ACGAATCACACTTTTACCACT	GTCTTACCGGGGCATTACTC	38.1	55.0	55.5	57.8	1605-1653 bp	1.0	53.5
trnG-UCC-trnM-CAU_p3	ACGAATCACACTTTTACCACT	GACTTATGTCTTACCGGGGC	38.1	55.0	55.5	57.8	1612-1660 bp	1.0	53.4
trnG-UCC-trnM-CAU_p4	ACGAATCACACTTTTACCACT	TACCGGGGCATTACTCTACC	38.1	55.0	55.5	58.3	1601-1649 bp	1.0	53.0
trnG-UCC-trnM-CAU_p5	GAACGAATCACACTTTTACCACT	TCTTACCGGGGCATTACTCT	39.1	50.0	57.3	57.8	1606-1654 bp	1.0	52.9