Hotspot

ycf3

LSC · 44031-46107 · 2077 bp

Back to Dalenia

Pi 0.00771

MarkerSeek score 61.90

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 2077
variable_sites: 16
pi: 0.00771
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	TCCGTTTTATTTGCCATTTGACT	TCCTTTGTTTGTGGGGAATCA	34.8	42.9	57.5	57.7	2200-2201 bp	1.0	42.4
ycf3_p2	TCCGTTTTATTTGCCATTTGAC	TCCTTTGTTTGTGGGGAATCA	36.4	42.9	56.1	57.7	2200-2201 bp	1.0	40.7
ycf3_p3	CCGTTTTATTTGCCATTTGACT	TCCTTTGTTTGTGGGGAATCA	36.4	42.9	56.1	57.7	2199-2200 bp	1.0	40.7
ycf3_p4	TCCGTTTTATTTGCCATTTGACT	ACAATTCCTTTGTTTGTGGGG	34.8	42.9	57.5	57.2	2205-2206 bp	1.0	40.7
ycf3_p5	TCCGTTTTATTTGCCATTTGACT	CCTTTGTTTGTGGGGAATCA	34.8	45.0	57.5	56.1	2199-2200 bp	1.0	40.7