Hotspot

atpF

LSC · 12020-13425 · 1406 bp

Back to Dalenia

Pi 0.00142

MarkerSeek score 59.60

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1406
variable_sites: 2
pi: 0.00142
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	CGTCGGCTTTAATGGTTACC	CGAGAAGAAATTCATAGAAATCGA	50.0	33.3	56.9	55.2	1600 bp	1.0	40.1
atpF_p2	CGTCGGCTTTAATGGTTACC	TCTGTAAAAGAAAGGAGATTGT	50.0	31.8	56.9	53.0	1502 bp	1.0	40.1
atpF_p3	TCGTCGGCTTTAATGGTTACC	CGAGAAGAAATTCATAGAAATCGA	47.6	33.3	58.4	55.2	1601 bp	1.0	40.1
atpF_p4	CGTCGGCTTTAATGGTTACC	CGAGAAGAAATTCATAGAAATCGAA	50.0	32.0	56.9	55.8	1600 bp	1.0	40.1
atpF_p5	CGTCGGCTTTAATGGTTACC	CGAGAAGAAATTCATAGAAATCG	50.0	34.8	56.9	53.8	1600 bp	1.0	40.1