Hotspot

rpl14-rps3

LSC · 82785-84516 · 1732 bp

Back to Dalenia

Pi 0.0029

MarkerSeek score 60.20

Primer available yes

Label rpl14-rps3

Feature Metadata

label_name: rpl14-rps3
feature_type: igs
region: LSC
length_bp: 1732
variable_sites: 5
pi: 0.0029
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rps3_p1	ACATCAATTCTCGAGCTCCG	CTCTACAAACCATTCGGGCT	50.0	50.0	57.8	57.6	1891-1894 bp	1.0	75.5
rpl14-rps3_p2	CGCGACATTCAAATGGGTTT	CTCTACAAACCATTCGGGCT	45.0	50.0	57.7	57.6	1863-1866 bp	1.0	74.4
rpl14-rps3_p3	TTACTCGCTCCGATGATTCG	CTCTACAAACCATTCGGGCT	50.0	50.0	57.6	57.6	1916-1919 bp	1.0	74.0
rpl14-rps3_p4	ACATCAATTCTCGAGCTCCG	CCTCTACAAACCATTCGGGC	50.0	55.0	57.8	58.6	1892-1895 bp	1.0	73.7
rpl14-rps3_p5	CGCGACATTCAAATGGGTTT	CCTCTACAAACCATTCGGGC	45.0	55.0	57.7	58.6	1864-1867 bp	1.0	72.7