Hotspot

rpl20-rps12

LSC · 70573-71422 · 850 bp

Back to Dalenia

Pi 0

MarkerSeek score 59.90

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 850
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	CTCGAATGAGCTCCACGAAA	ACAGCCAATCCGAAATGTCA	50.0	45.0	58.0	57.8	1008-1018 bp	1.0	79.2
rpl20-rps12_p2	TCGAATGAGCTCCACGAAAG	ACAGCCAATCCGAAATGTCA	50.0	45.0	58.0	57.8	1007-1017 bp	1.0	79.2
rpl20-rps12_p3	CTCGAATGAGCTCCACGAAA	ACAAGACAGCCAATCCGAAA	50.0	45.0	58.0	57.7	1013-1023 bp	1.0	78.6
rpl20-rps12_p4	TCGAATGAGCTCCACGAAAG	ACAAGACAGCCAATCCGAAA	50.0	45.0	58.0	57.7	1012-1022 bp	1.0	78.6
rpl20-rps12_p5	TCGAATGAGCTCCACGAAAG	CACAAGACAGCCAATCCGAA	50.0	50.0	58.0	58.5	1013-1023 bp	1.0	76.9