Hotspot

psbE-petL

LSC · 66204-67354 · 1151 bp

Back to Dalenia

Pi 0.00349

MarkerSeek score 61.20

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1151
variable_sites: 4
pi: 0.00349
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	TGGGTTGTGCTCATTCAATTG	50.0	42.9	57.8	57.3	1298-1337 bp	1.0	66.0
psbE-petL_p2	ACGAATAACCAACCCGCAAT	TGGGTTGTGCTCATTCAATTG	45.0	42.9	58.2	57.3	1384-1423 bp	1.0	65.8
psbE-petL_p3	TGCTGACGAATAACCAACCC	TGGGTTGTGCTCATTCAATTG	50.0	42.9	58.2	57.3	1389-1428 bp	1.0	65.7
psbE-petL_p4	GTTCTCCTGTGCTTCCAGAC	TGGGTTGTGCTCATTCAATTG	55.0	42.9	58.2	57.3	1293-1332 bp	1.0	65.7
psbE-petL_p5	TCTCCTGTGCTTCCAGACAT	TGGGTTGTGCTCATTCAATTG	50.0	42.9	58.4	57.3	1291-1330 bp	1.0	64.4