Hotspot

psaI-ycf4

LSC · 61271-61695 · 425 bp

Back to Dalenia

Pi 0.0118

MarkerSeek score 61.90

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 425
variable_sites: 5
pi: 0.0118
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	ATTTCCGGCAATTGCAATGG	TGTTCGGATCGCAAACTCAT	45.0	45.0	58.0	57.9	506-509 bp	1.0	79.8
psaI-ycf4_p2	GGCAATTGCAATGGCTTCTT	TGTTCGGATCGCAAACTCAT	45.0	45.0	57.9	57.9	500-503 bp	1.0	79.2
psaI-ycf4_p3	ATTTCCGGCAATTGCAATGG	TCTGTTCGGATCGCAAACTC	45.0	50.0	58.0	58.3	508-511 bp	1.0	78.2
psaI-ycf4_p4	ATTTCCGGCAATTGCAATGG	AAGGATCAAGGCCCAGAAGA	45.0	50.0	58.0	58.3	570-573 bp	1.0	77.9
psaI-ycf4_p5	ATTTCCGGCAATTGCAATGG	AGGATCAAGGCCCAGAAGAA	45.0	50.0	58.0	58.3	569-572 bp	1.0	77.9