Hotspot

trnD-GUC-trnY-GUA

LSC · 31232-31619 · 388 bp

Back to Dalenia

Pi 0.00515
MarkerSeek score 49.00
Primer available yes
Label trnD-GUC-trnY-GUA

Feature Metadata

label_name
trnD-GUC-trnY-GUA
feature_type
igs
region
LSC
length_bp
388
variable_sites
2
pi
0.00515
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-trnY-GUA_p1 GCGGTGCTCTAACCAATTGA TGGCAATATGTCTACGCTGG 50.0 50.0 58.6 57.8 459 bp 1.0 74.0
trnD-GUC-trnY-GUA_p2 GCGGTGCTCTAACCAATTGA GGCAATATGTCTACGCTGGT 50.0 50.0 58.6 57.8 458 bp 1.0 74.0
trnD-GUC-trnY-GUA_p3 TTTATTCCGTCGGGACTGAC TGGCAATATGTCTACGCTGG 50.0 50.0 57.3 57.8 512 bp 1.0 73.1
trnD-GUC-trnY-GUA_p4 TTTATTCCGTCGGGACTGAC GGCAATATGTCTACGCTGGT 50.0 50.0 57.3 57.8 511 bp 1.0 73.1
trnD-GUC-trnY-GUA_p5 GCGGTGCTCTAACCAATTGA GGGGACGGACTGTAAATTCG 50.0 55.0 58.6 58.4 480 bp 1.0 73.1