Hotspot

atpH-atpI

LSC · 14293-15240 · 948 bp

Back to Dalenia

Pi 0.00107

MarkerSeek score 60.50

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 948
variable_sites: 1
pi: 0.00107
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TAGCCGCCGCTTATATAGGA	45.0	50.0	58.0	57.9	1013-1024 bp	1.0	79.6
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACTTTAGCCGCC	45.0	45.0	58.0	58.1	1025-1036 bp	1.0	79.5
atpH-atpI_p3	GAAGCAAGTCCGACAGCTAA	TAGCCGCCGCTTATATAGGA	50.0	50.0	57.9	57.9	1043-1054 bp	1.0	79.2
atpH-atpI_p4	GAAGCAAGTCCGACAGCTAA	TTTTTGCAACTTTAGCCGCC	50.0	45.0	57.9	58.1	1055-1066 bp	1.0	79.1
atpH-atpI_p5	AATAACGGAAGCGGCAGAAA	TAGCCGCCGCTTATATAGGA	45.0	50.0	58.2	57.9	1014-1025 bp	1.0	78.4