Hotspot

rps16-trnQ-UUG

LSC · 5742-6914 · 1173 bp

Back to Dalenia

Pi 0.00256

MarkerSeek score 60.30

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1173
variable_sites: 3
pi: 0.00256
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGAATCCACGAACGATCCC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.7	58.1	1272-1273 bp	1.0	77.2
rps16-trnQ-UUG_p2	CGTTGCTTTCTACCACATCG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.2	58.1	1237-1238 bp	1.0	73.3
rps16-trnQ-UUG_p3	AAGAATCCACGAACGATCCC	TTCGGAGGTTCGAATCCTTC	50.0	50.0	57.7	57.3	1276-1277 bp	1.0	72.4
rps16-trnQ-UUG_p4	AAGAATCCACGAACGATCCC	TAAGGCAACGGGTTTTGGTC	50.0	50.0	57.7	58.7	1302-1303 bp	1.0	72.3
rps16-trnQ-UUG_p5	AAGAATCCACGAACGATCCC	AGAAAGACAAATGGGGCGTG	50.0	50.0	57.7	58.8	1331-1332 bp	1.0	71.7