primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGAATCCACGAACGATCCC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.7	58.1	0.1	16.9	0.387225	5742	6914	1272-1273	1.000000	77.2
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	CGTTGCTTTCTACCACATCG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.2	58.1	0.0	16.9	0.863824	5742	6914	1237-1238	1.000000	73.3
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGAATCCACGAACGATCCC	TTCGGAGGTTCGAATCCTTC	20	20	50.0	50.0	57.7	57.3	0.1	16.9	0.978597	5742	6914	1276-1277	1.000000	72.4
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	AAGAATCCACGAACGATCCC	TAAGGCAACGGGTTTTGGTC	20	20	50.0	50.0	57.7	58.7	0.1	0.0	0.990617	5742	6914	1302-1303	1.000000	72.3
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AAGAATCCACGAACGATCCC	AGAAAGACAAATGGGGCGTG	20	20	50.0	50.0	57.7	58.8	0.1	0.0	1.063557	5742	6914	1331-1332	1.000000	71.7
trnS-GCU-trnR-UCU_p1	trnS-GCU-trnR-UCU	1	ACGGAAAGAGAGGGATTCGA	AGAACTTGCGTCCAATAGGA	20	20	50.0	45.0	58.2	56.6	0.0	0.0	1.594719	8183	10018	2000-2004	1.000000	67.6
trnS-GCU-trnR-UCU_p2	trnS-GCU-trnR-UCU	2	TAGCAATCCGACGCTTTAGG	AGAACTTGCGTCCAATAGGA	20	20	50.0	45.0	57.8	56.6	0.0	0.0	1.662324	8183	10018	1949-1953	1.000000	67.1
trnS-GCU-trnR-UCU_p3	trnS-GCU-trnR-UCU	3	ACGGAAAGAGAGGGATTCGA	ACATAGAAGAACTTGCGTCCA	20	21	50.0	42.9	58.2	57.3	0.0	0.0	1.829404	8183	10018	2007-2011	1.000000	65.8
trnS-GCU-trnR-UCU_p4	trnS-GCU-trnR-UCU	4	ACGGAAAGAGAGGGATTCGA	AAGAACTTGCGTCCAATAGGA	20	21	50.0	42.9	58.2	57.3	0.0	0.0	1.847178	8183	10018	2001-2005	1.000000	65.6
trnS-GCU-trnR-UCU_p5	trnS-GCU-trnR-UCU	5	TAGCAATCCGACGCTTTAGG	ACATAGAAGAACTTGCGTCCA	20	21	50.0	42.9	57.8	57.3	0.0	0.0	1.897009	8183	10018	1956-1960	1.000000	65.2
atpF_p1	atpF	1	CGTCGGCTTTAATGGTTACC	CGAGAAGAAATTCATAGAAATCGA	20	24	50.0	33.3	56.9	55.2	0.0	0.0	7.944964	12020	13425	1600-1600	1.000000	40.1
atpF_p2	atpF	2	CGTCGGCTTTAATGGTTACC	TCTGTAAAAGAAAGGAGATTGT	20	22	50.0	31.8	56.9	53.0	0.0	0.0	8.078375	12020	13425	1502-1502	1.000000	40.1
atpF_p3	atpF	3	TCGTCGGCTTTAATGGTTACC	CGAGAAGAAATTCATAGAAATCGA	21	24	47.6	33.3	58.4	55.2	0.0	0.0	8.224990	12020	13425	1601-1601	1.000000	40.1
atpF_p4	atpF	4	CGTCGGCTTTAATGGTTACC	CGAGAAGAAATTCATAGAAATCGAA	20	25	50.0	32.0	56.9	55.8	0.0	0.0	8.272125	12020	13425	1600-1600	1.000000	40.1
atpF_p5	atpF	5	CGTCGGCTTTAATGGTTACC	CGAGAAGAAATTCATAGAAATCG	20	23	50.0	34.8	56.9	53.8	0.0	0.0	8.335492	12020	13425	1600-1600	1.000000	40.1
atpH-atpI_p1	atpH-atpI	1	ATAACGGAAGCGGCAGAAAT	TAGCCGCCGCTTATATAGGA	20	20	45.0	50.0	58.0	57.9	1.4	4.5	0.171668	14293	15240	1013-1024	1.000000	79.6
atpH-atpI_p2	atpH-atpI	2	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACTTTAGCCGCC	20	20	45.0	45.0	58.0	58.1	1.4	0.0	0.179004	14293	15240	1025-1036	1.000000	79.5
atpH-atpI_p3	atpH-atpI	3	GAAGCAAGTCCGACAGCTAA	TAGCCGCCGCTTATATAGGA	20	20	50.0	50.0	57.9	57.9	16.1	4.5	0.213567	14293	15240	1043-1054	1.000000	79.2
atpH-atpI_p4	atpH-atpI	4	GAAGCAAGTCCGACAGCTAA	TTTTTGCAACTTTAGCCGCC	20	20	50.0	45.0	57.9	58.1	16.1	0.0	0.220903	14293	15240	1055-1066	1.000000	79.1
atpH-atpI_p5	atpH-atpI	5	AATAACGGAAGCGGCAGAAA	TAGCCGCCGCTTATATAGGA	20	20	45.0	50.0	58.2	57.9	1.4	4.5	0.317207	14293	15240	1014-1025	1.000000	78.4
trnD-GUC-trnY-GUA_p1	trnD-GUC-trnY-GUA	1	GCGGTGCTCTAACCAATTGA	TGGCAATATGTCTACGCTGG	20	20	50.0	50.0	58.6	57.8	7.1	0.0	0.789957	31232	31619	459-459	1.000000	74.0
trnD-GUC-trnY-GUA_p2	trnD-GUC-trnY-GUA	2	GCGGTGCTCTAACCAATTGA	GGCAATATGTCTACGCTGGT	20	20	50.0	50.0	58.6	57.8	7.1	0.0	0.791509	31232	31619	458-458	1.000000	74.0
trnD-GUC-trnY-GUA_p3	trnD-GUC-trnY-GUA	3	TTTATTCCGTCGGGACTGAC	TGGCAATATGTCTACGCTGG	20	20	50.0	50.0	57.3	57.8	12.3	0.0	0.899130	31232	31619	512-512	1.000000	73.1
trnD-GUC-trnY-GUA_p4	trnD-GUC-trnY-GUA	4	TTTATTCCGTCGGGACTGAC	GGCAATATGTCTACGCTGGT	20	20	50.0	50.0	57.3	57.8	12.3	0.0	0.900682	31232	31619	511-511	1.000000	73.1
trnD-GUC-trnY-GUA_p5	trnD-GUC-trnY-GUA	5	GCGGTGCTCTAACCAATTGA	GGGGACGGACTGTAAATTCG	20	20	50.0	55.0	58.6	58.4	7.1	0.0	0.902511	31232	31619	480-480	1.000000	73.1
trnY-GUA-trnE-UUC_p1	trnY-GUA-trnE-UUC	1	CGAGCGGATCAGTGAATTCT	AAGTATGCCCCCATCGTCTA	20	20	50.0	50.0	57.8	57.9	7.1	0.0	0.313017	31704	31762	242-242	1.000000	78.2
trnY-GUA-trnE-UUC_p2	trnY-GUA-trnE-UUC	2	CGAGCGGATCAGTGAATTCT	TAATATGATGGCGGTTGGGC	20	20	50.0	50.0	57.8	58.1	7.1	0.0	0.311620	31704	31762	262-262	1.000000	78.1
trnY-GUA-trnE-UUC_p3	trnY-GUA-trnE-UUC	3	CCAGCGTAGACATATTGCCA	AAGTATGCCCCCATCGTCTA	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.336052	31704	31762	202-202	1.000000	78.1
trnY-GUA-trnE-UUC_p4	trnY-GUA-trnE-UUC	4	CCAGCGTAGACATATTGCCA	TAATATGATGGCGGTTGGGC	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.334655	31704	31762	222-222	1.000000	78.0
trnY-GUA-trnE-UUC_p5	trnY-GUA-trnE-UUC	5	ACCAGCGTAGACATATTGCC	TAATATGATGGCGGTTGGGC	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.336206	31704	31762	223-223	1.000000	78.0
ycf3_p1	ycf3	1	TCCGTTTTATTTGCCATTTGACT	TCCTTTGTTTGTGGGGAATCA	23	21	34.8	42.9	57.5	57.7	0.0	0.0	4.781643	44031	46107	2200-2201	1.000000	42.4
ycf3_p2	ycf3	2	TCCGTTTTATTTGCCATTTGAC	TCCTTTGTTTGTGGGGAATCA	22	21	36.4	42.9	56.1	57.7	0.0	0.0	5.174085	44031	46107	2200-2201	1.000000	40.7
ycf3_p3	ycf3	3	CCGTTTTATTTGCCATTTGACT	TCCTTTGTTTGTGGGGAATCA	22	21	36.4	42.9	56.1	57.7	0.0	0.0	5.183512	44031	46107	2199-2200	1.000000	40.7
ycf3_p4	ycf3	4	TCCGTTTTATTTGCCATTTGACT	ACAATTCCTTTGTTTGTGGGG	23	21	34.8	42.9	57.5	57.2	0.0	0.0	5.297397	44031	46107	2205-2206	1.000000	40.7
ycf3_p5	ycf3	5	TCCGTTTTATTTGCCATTTGACT	CCTTTGTTTGTGGGGAATCA	23	20	34.8	45.0	57.5	56.1	0.0	0.0	5.344389	44031	46107	2199-2200	1.000000	40.7
rps4-trnT-UGU_p1	rps4-trnT-UGU	1	TAGACCTGGTAAAGCCCCAA	CTCAGAGGTTAGAGCATCGC	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.180509	47553	47954	528-528	1.000000	79.3
rps4-trnT-UGU_p2	rps4-trnT-UGU	2	TTAGACCTGGTAAAGCCCCA	CTCAGAGGTTAGAGCATCGC	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.180509	47553	47954	529-529	1.000000	79.3
rps4-trnT-UGU_p3	rps4-trnT-UGU	3	AAAATCGCTTCCGGCTCTAG	CTCAGAGGTTAGAGCATCGC	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.191415	47553	47954	561-561	1.000000	79.2
rps4-trnT-UGU_p4	rps4-trnT-UGU	4	TGAAACGAGGCCCCCTATAA	CTCAGAGGTTAGAGCATCGC	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.250432	47553	47954	493-493	1.000000	78.8
rps4-trnT-UGU_p5	rps4-trnT-UGU	5	TTGAAACGAGGCCCCCTATA	CTCAGAGGTTAGAGCATCGC	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.250432	47553	47954	494-494	1.000000	78.8
trnL-UAA_p1	trnL-UAA	1	ACACTGCTTTGATTTCAGCA	AGGATAAAGCATTAACGAGTCA	20	22	40.0	36.4	56.2	55.3	0.0	0.0	6.562856	48840	49462	688-690	1.000000	40.1
trnL-UAA_p2	trnL-UAA	2	ACACTGCTTTGATTTCAGCA	AGCATTAACGAGTCAAATGT	20	20	40.0	35.0	56.2	53.2	0.0	10.1	6.618595	48840	49462	681-683	1.000000	40.1
trnL-UAA_p3	trnL-UAA	3	ACACTGCTTTGATTTCAGCA	AAGCATTAACGAGTCAAATGT	20	21	40.0	33.3	56.2	54.1	0.0	10.1	6.721940	48840	49462	682-684	1.000000	40.1
trnL-UAA_p4	trnL-UAA	4	ACACTGCTTTGATTTCAGCA	AGCATTAACGAGTCAAATGTT	20	21	40.0	33.3	56.2	54.1	0.0	10.1	6.721940	48840	49462	681-683	1.000000	40.1
trnL-UAA_p5	trnL-UAA	5	ACACTGCTTTGATTTCAGCA	AGGATAAAGCATTAACGAGTCAA	20	23	40.0	34.8	56.2	56.0	0.0	0.0	6.824138	48840	49462	688-690	1.000000	40.1
trnC-GCA-ndhJ_p1	trnC-GCA-ndhJ	1	GGGATAGCTCAGCTGGTAGA	ACTCATCCACGACTGAAACG	20	20	55.0	50.0	58.0	57.9	10.3	0.0	0.068498	49811	50661	1015-1029	1.000000	80.7
trnC-GCA-ndhJ_p2	trnC-GCA-ndhJ	2	GGGATAGCTCAGCTGGTAGA	CTCATCCACGACTGAAACGT	20	20	55.0	50.0	58.0	57.9	10.3	0.0	0.068498	49811	50661	1014-1028	1.000000	80.7
trnC-GCA-ndhJ_p3	trnC-GCA-ndhJ	3	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	20	20	55.0	50.0	58.0	58.1	10.3	0.0	0.089643	49811	50661	979-993	1.000000	80.6
trnC-GCA-ndhJ_p4	trnC-GCA-ndhJ	4	CCTCGTGTCACCAGTTCAAA	ACTCATCCACGACTGAAACG	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.117888	49811	50661	979-993	1.000000	80.4
trnC-GCA-ndhJ_p5	trnC-GCA-ndhJ	5	CCTCGTGTCACCAGTTCAAA	CTCATCCACGACTGAAACGT	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.117888	49811	50661	978-992	1.000000	80.4
psaI-ycf4_p1	psaI-ycf4	1	ATTTCCGGCAATTGCAATGG	TGTTCGGATCGCAAACTCAT	20	20	45.0	45.0	58.0	57.9	30.3	5.5	0.120310	61271	61695	506-509	1.000000	79.8
psaI-ycf4_p2	psaI-ycf4	2	GGCAATTGCAATGGCTTCTT	TGTTCGGATCGCAAACTCAT	20	20	45.0	45.0	57.9	57.9	32.4	5.5	0.196843	61271	61695	500-503	1.000000	79.2
psaI-ycf4_p3	psaI-ycf4	3	ATTTCCGGCAATTGCAATGG	TCTGTTCGGATCGCAAACTC	20	20	45.0	50.0	58.0	58.3	30.3	5.5	0.316990	61271	61695	508-511	1.000000	78.2
psaI-ycf4_p4	psaI-ycf4	4	ATTTCCGGCAATTGCAATGG	AAGGATCAAGGCCCAGAAGA	20	20	45.0	50.0	58.0	58.3	30.3	0.0	0.353236	61271	61695	570-573	1.000000	77.9
psaI-ycf4_p5	psaI-ycf4	5	ATTTCCGGCAATTGCAATGG	AGGATCAAGGCCCAGAAGAA	20	20	45.0	50.0	58.0	58.3	30.3	0.0	0.353236	61271	61695	569-572	1.000000	77.9
psbE-petL_p1	psbE-petL	1	AGAACGTTCTCCTGTGCTTC	TGGGTTGTGCTCATTCAATTG	20	21	50.0	42.9	57.8	57.3	24.9	0.0	1.820338	66204	67354	1298-1337	1.000000	66.0
psbE-petL_p2	psbE-petL	2	ACGAATAACCAACCCGCAAT	TGGGTTGTGCTCATTCAATTG	20	21	45.0	42.9	58.2	57.3	0.0	0.0	1.835015	66204	67354	1384-1423	1.000000	65.8
psbE-petL_p3	psbE-petL	3	TGCTGACGAATAACCAACCC	TGGGTTGTGCTCATTCAATTG	20	21	50.0	42.9	58.2	57.3	0.0	0.0	1.847920	66204	67354	1389-1428	1.000000	65.7
psbE-petL_p4	psbE-petL	4	GTTCTCCTGTGCTTCCAGAC	TGGGTTGTGCTCATTCAATTG	20	21	55.0	42.9	58.2	57.3	0.9	0.0	1.861926	66204	67354	1293-1332	1.000000	65.7
psbE-petL_p5	psbE-petL	5	TCTCCTGTGCTTCCAGACAT	TGGGTTGTGCTCATTCAATTG	20	21	50.0	42.9	58.4	57.3	0.9	0.0	2.014673	66204	67354	1291-1330	1.000000	64.4
rpl20-rps12_p1	rpl20-rps12	1	CTCGAATGAGCTCCACGAAA	ACAGCCAATCCGAAATGTCA	20	20	50.0	45.0	58.0	57.8	20.9	0.0	0.202535	70573	71422	1008-1018	1.000000	79.2
rpl20-rps12_p2	rpl20-rps12	2	TCGAATGAGCTCCACGAAAG	ACAGCCAATCCGAAATGTCA	20	20	50.0	45.0	58.0	57.8	19.2	0.0	0.202535	70573	71422	1007-1017	1.000000	79.2
rpl20-rps12_p3	rpl20-rps12	3	CTCGAATGAGCTCCACGAAA	ACAAGACAGCCAATCCGAAA	20	20	50.0	45.0	58.0	57.7	20.9	0.0	0.274060	70573	71422	1013-1023	1.000000	78.6
rpl20-rps12_p4	rpl20-rps12	4	TCGAATGAGCTCCACGAAAG	ACAAGACAGCCAATCCGAAA	20	20	50.0	45.0	58.0	57.7	19.2	0.0	0.274060	70573	71422	1012-1022	1.000000	78.6
rpl20-rps12_p5	rpl20-rps12	5	TCGAATGAGCTCCACGAAAG	CACAAGACAGCCAATCCGAA	20	20	50.0	50.0	58.0	58.5	19.2	0.0	0.481040	70573	71422	1013-1023	1.000000	76.9
rpl14-rps3_p1	rpl14-rps3	1	ACATCAATTCTCGAGCTCCG	CTCTACAAACCATTCGGGCT	20	20	50.0	50.0	57.8	57.6	7.8	0.0	0.619159	82785	84516	1891-1894	1.000000	75.5
rpl14-rps3_p2	rpl14-rps3	2	CGCGACATTCAAATGGGTTT	CTCTACAAACCATTCGGGCT	20	20	45.0	50.0	57.7	57.6	0.0	0.0	0.749351	82785	84516	1863-1866	1.000000	74.4
rpl14-rps3_p3	rpl14-rps3	3	TTACTCGCTCCGATGATTCG	CTCTACAAACCATTCGGGCT	20	20	50.0	50.0	57.6	57.6	0.0	0.0	0.804269	82785	84516	1916-1919	1.000000	74.0
rpl14-rps3_p4	rpl14-rps3	4	ACATCAATTCTCGAGCTCCG	CCTCTACAAACCATTCGGGC	20	20	50.0	55.0	57.8	58.6	7.8	0.0	0.836381	82785	84516	1892-1895	1.000000	73.7
rpl14-rps3_p5	rpl14-rps3	5	CGCGACATTCAAATGGGTTT	CCTCTACAAACCATTCGGGC	20	20	45.0	55.0	57.7	58.6	0.0	0.0	0.966573	82785	84516	1864-1867	1.000000	72.7
ndhF-rpl32_p1	ndhF-rpl32	1	TGTTGCCATTGGAAAAACGA	TGAAAAAGCCTTCAACGCTG	20	20	40.0	45.0	56.7	57.5	6.9	0.0	1.747675	114842	115724	1074-1081	1.000000	66.7
ndhF-rpl32_p2	ndhF-rpl32	2	TGTTGCCATTGGAAAAACGA	GAAAAAGCCTTCAACGCTGT	20	20	40.0	45.0	56.7	57.5	6.9	0.0	1.749367	114842	115724	1073-1080	1.000000	66.7
ndhF-rpl32_p3	ndhF-rpl32	3	TTGTTGCCATTGGAAAAACGA	TGAAAAAGCCTTCAACGCTG	21	20	38.1	45.0	57.5	57.5	6.9	0.0	2.017259	114842	115724	1075-1082	1.000000	64.5
ndhF-rpl32_p4	ndhF-rpl32	4	TTGTTGCCATTGGAAAAACGA	GAAAAAGCCTTCAACGCTGT	21	20	38.1	45.0	57.5	57.5	6.9	0.0	2.018951	114842	115724	1074-1081	1.000000	64.5
ndhF-rpl32_p5	ndhF-rpl32	5	TGTTGCCATTGGAAAAACGAT	TGAAAAAGCCTTCAACGCTG	21	20	38.1	45.0	57.3	57.5	6.9	0.0	2.232761	114842	115724	1074-1081	1.000000	62.8
ndhH_p1	ndhH	1	AAAGCATCCCTATGATCCCA	ACTTCTTTTCGTTTTAAGCAGTTCA	20	25	45.0	32.0	55.9	58.2	0.0	0.0	7.211804	123887	125068	1325-1325	1.000000	40.1
ndhH_p2	ndhH	2	AAAGCATCCCTATGATCCCA	TCTTTTCGTTTTAAGCAGTTCA	20	22	45.0	31.8	55.9	54.8	0.0	0.0	7.237276	123887	125068	1322-1322	1.000000	40.1
ndhH_p3	ndhH	3	AAAGCATCCCTATGATCCCA	ACTTCTTTTCGTTTTAAGCAGT	20	22	45.0	31.8	55.9	54.8	0.0	0.0	7.253019	123887	125068	1325-1325	1.000000	40.1
ndhH_p4	ndhH	4	AAAGCATCCCTATGATCCCA	CTTCTTTTCGTTTTAAGCAGTTCA	20	24	45.0	33.3	55.9	56.7	0.0	0.0	7.406558	123887	125068	1324-1324	1.000000	40.1
ndhH_p5	ndhH	5	AAAGCATCCCTATGATCCCA	ACTTCTTTTCGTTTTAAGCAGTTC	20	24	45.0	33.3	55.9	56.7	0.0	0.0	7.408437	123887	125068	1325-1325	1.000000	40.1