Hotspot

ycf1

SSC · 111187-116514 · 5328 bp

Back to Dalea

Pi 0.407
MarkerSeek score 74.50
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5328
variable_sites
1833
pi
0.407
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.85
markerseek_score
74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 AACAGAATACGCGGATCGAG AGCCAAAGCCTTACAGCAAT 50.0 45.0 57.9 58.1 748 bp 0.5 58.5
ycf1_p2 TAACCCGTGGATCATACCCA AGCCAAAGCCTTACAGCAAT 50.0 45.0 57.8 58.1 916 bp 0.5 58.0
ycf1_p3 AACAGAATACGCGGATCGAG CCTACGACGTCTAGCGGATA 50.0 55.0 57.9 58.2 640 bp 0.5 57.3
ycf1_p4 ACAGGCCTTTCGTTGATTCA AGCCAAAGCCTTACAGCAAT 45.0 45.0 57.7 58.1 300 bp 0.5 57.3
ycf1_p5 TCACAGGCCTTTCGTTGATT AGCCAAAGCCTTACAGCAAT 45.0 45.0 57.7 58.1 302 bp 0.5 57.3