Hotspot

ndhI

SSC · 120866-121348 · 483 bp

Back to Dalea

Pi 0.482
MarkerSeek score 78.60
Primer available yes
Label ndhI

Feature Metadata

label_name
ndhI
feature_type
gene
region
SSC
length_bp
483
variable_sites
200
pi
0.482
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.86
markerseek_score
78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhI_p1 ACAGTTCTTTACTTGGGCGG GTGCTGCAGCTACAAAATGG 50.0 50.0 58.1 58.0 1501 bp 0.5 59.0
ndhI_p2 CAGTTCTTTACTTGGGCGGT GTGCTGCAGCTACAAAATGG 50.0 50.0 58.1 58.0 1500 bp 0.5 59.0
ndhI_p3 ACAGTTCTTTACTTGGGCGG CAAGAACCAGTCCCAACGAA 50.0 50.0 58.1 58.0 1447 bp 0.5 58.8
ndhI_p4 CAGTTCTTTACTTGGGCGGT CAAGAACCAGTCCCAACGAA 50.0 50.0 58.1 58.0 1446 bp 0.5 58.8
ndhI_p5 CAGTTCTTTACTTGGGCGGT GGACCAATGTCATTCCCCTC 50.0 55.0 58.1 57.9 1174 bp 0.5 58.7