Hotspot

ndhF

SSC · 127881-130106 · 2226 bp

Back to Dalea

Pi 0.468
MarkerSeek score 78.40
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
SSC
length_bp
2226
variable_sites
851
pi
0.468
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.82
markerseek_score
78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 GGTATTTGCCCATTTTGACT GAGCTCGGGTTATGCAAGAA 40.0 50.0 54.1 58.0 2754 bp 0.5 28.6
ndhF_p2 GGTATTTGCCCATTTTGACT AGCTCGGGTTATGCAAGAAG 40.0 50.0 54.1 58.0 2753 bp 0.5 28.6
ndhF_p3 GGTATTTGCCCATTTTGACT TGAATTCGGTCGTTGTGGTT 40.0 45.0 54.1 58.0 2835 bp 0.5 28.5
ndhF_p4 GGTATTTGCCCATTTTGACT GCAAGAAGGGACCGAAAAGA 40.0 50.0 54.1 58.1 2741 bp 0.5 28.0
ndhF_p5 GGTATTTGCCCATTTTGACT GGGCCCTCTTATCTCTTCCT 40.0 55.0 54.1 57.9 2779 bp 0.5 27.9