Hotspot

rpl32-ndhF

SSC · 127251-127880 · 630 bp

Back to Dalea

Pi 0.372
MarkerSeek score 79.90
Primer available yes
Label rpl32-ndhF

Feature Metadata

label_name
rpl32-ndhF
feature_type
igs
region
SSC
length_bp
630
variable_sites
192
pi
0.372
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.82
markerseek_score
79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-ndhF_p1 TGACCTCAATGCAGGAATGG GCCCACATACGTCGAAGATT 50.0 50.0 57.9 58.1 1280 bp 0.5 58.4
rpl32-ndhF_p2 TGACCTCAATGCAGGAATGG AGGAAAAGCCCACATACGTC 50.0 50.0 57.9 57.9 1287 bp 0.5 58.1
rpl32-ndhF_p3 TGACCTCAATGCAGGAATGG ACATGAAGGGGGAATTGAGC 50.0 50.0 57.9 57.9 1901 bp 0.5 57.7
rpl32-ndhF_p4 TGACCTCAATGCAGGAATGG AAAAGCCCACATACGTCGAA 50.0 45.0 57.9 57.8 1284 bp 0.5 57.6
rpl32-ndhF_p5 TGACCTCAATGCAGGAATGG TAAACCGAAATCCCCCACAC 50.0 50.0 57.9 57.8 1725 bp 0.5 57.3