Hotspot

psaC-ccsA

SSC · 123402-125283 · 1882 bp

Back to Dalea

Pi 0.472
MarkerSeek score 79.20
Primer available yes
Label psaC-ccsA

Feature Metadata

label_name
psaC-ccsA
feature_type
igs
region
SSC
length_bp
1882
variable_sites
727
pi
0.472
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.82
markerseek_score
79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaC-ccsA_p1 AAACAACTCGAAGCATGGGT GAGGCGTGGGGATCATATTG 45.0 55.0 58.0 58.2 2139 bp 0.5 58.4
psaC-ccsA_p2 AAACAACTCGAAGCATGGGT GCTTTGCTACTTCGGGTCTT 45.0 50.0 58.0 58.2 2555 bp 0.5 58.3
psaC-ccsA_p3 AGATGTGAATCCGCTTGTCC GAGGCGTGGGGATCATATTG 50.0 55.0 58.0 58.2 2197 bp 0.5 58.3
psaC-ccsA_p4 GGCATGAAACAACTCGAAGC GAGGCGTGGGGATCATATTG 50.0 55.0 58.0 58.2 2145 bp 0.5 58.3
psaC-ccsA_p5 AGATGTGAATCCGCTTGTCC GCTTTGCTACTTCGGGTCTT 50.0 50.0 58.0 58.2 2613 bp 0.5 58.2