Hotspot

ndhI-ndhG

SSC · 121349-121824 · 476 bp

Back to Dalea

Pi 0.43
MarkerSeek score 79.80
Primer available yes
Label ndhI-ndhG

Feature Metadata

label_name
ndhI-ndhG
feature_type
igs
region
SSC
length_bp
476
variable_sites
185
pi
0.43
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.90
markerseek_score
79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhI-ndhG_p1 TGGTCAACAAACAGTCCGAG GTGCTGCAGCTACAAAATGG 50.0 50.0 58.1 58.0 1111 bp 0.5 59.5
ndhI-ndhG_p2 TGGTCAACAAACAGTCCGAG CAAGAACCAGTCCCAACGAA 50.0 50.0 58.1 58.0 1057 bp 0.5 59.3
ndhI-ndhG_p3 AATCAAATTGCTTTGGGCCG GTGCTGCAGCTACAAAATGG 45.0 50.0 57.9 58.0 732 bp 0.5 59.1
ndhI-ndhG_p4 TGGTCAACAAACAGTCCGAG AACGGTTCTGGCAAATCCAT 50.0 45.0 58.1 58.1 947 bp 0.5 59.0
ndhI-ndhG_p5 TGGTCAACAAACAGTCCGAG ATGGAACGGTTCTGGCAAAT 50.0 45.0 58.1 58.1 951 bp 0.5 59.0