Hotspot

ycf1-rps15

SSC · 116515-116928 · 414 bp

Back to Dalea

Pi 0.426
MarkerSeek score 78.20
Primer available yes
Label ycf1-rps15

Feature Metadata

label_name
ycf1-rps15
feature_type
igs
region
SSC
length_bp
414
variable_sites
158
pi
0.426
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.90
markerseek_score
78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1-rps15_p1 ATTGCTGTAAGGCTTTGGCT AACACCATGCATTGATGGGT 45.0 45.0 58.1 58.1 1060 bp 0.5 59.0
ycf1-rps15_p2 ATTGCTGTAAGGCTTTGGCT TGCCATAAAAGGTCCCAACC 45.0 50.0 58.1 58.1 1354 bp 0.5 58.9
ycf1-rps15_p3 ATTGCTGTAAGGCTTTGGCT ATTTCCCAACAGTTCCGGTC 45.0 50.0 58.1 58.1 1258 bp 0.5 58.7
ycf1-rps15_p4 ATTGCTGTAAGGCTTTGGCT CCGATCCGTGGTTTTCTTCT 45.0 50.0 58.1 57.9 678 bp 0.5 58.7
ycf1-rps15_p5 ATTGCTGTAAGGCTTTGGCT TAATCGTTGCCGTTTTCCCA 45.0 45.0 58.1 58.1 803 bp 0.5 58.6