Hotspot

psbZ-trnG-GCC

LSC · 41144-41708 · 565 bp

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Pi 0.0412
MarkerSeek score 79.50
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
565
variable_sites
23
pi
0.0412
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 CTTCTCCTGATGGTTGGTCG CGTCTTCTCCTTGGCAAAGA 55.0 50.0 58.0 57.8 716-719 bp 1.0 81.9
psbZ-trnG-GCC_p2 TTGCTTCTCCTGATGGTTGG CGTCTTCTCCTTGGCAAAGA 50.0 50.0 57.8 57.8 719-722 bp 1.0 80.3
psbZ-trnG-GCC_p3 TCCTGATGGTTGGTCGAGTA CGTCTTCTCCTTGGCAAAGA 50.0 50.0 57.8 57.8 712-715 bp 1.0 80.2
psbZ-trnG-GCC_p4 GCTTCTCCTGATGGTTGGTC CGTCTTCTCCTTGGCAAAGA 55.0 50.0 58.3 57.8 717-720 bp 1.0 79.9
psbZ-trnG-GCC_p5 TTCTCCTGATGGTTGGTCGA CGTCTTCTCCTTGGCAAAGA 50.0 50.0 58.4 57.8 715-718 bp 1.0 79.1