Hotspot

atpF-atpH

LSC · 17605-18248 · 644 bp

Back to Dalea

Pi 0.0333
MarkerSeek score 79.90
Primer available yes
Label atpF-atpH

Feature Metadata

label_name
atpF-atpH
feature_type
igs
region
LSC
length_bp
644
variable_sites
21
pi
0.0333
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF-atpH_p1 CCAGTAACCCAAGCAAAGGA GGCTGGTTGTAGCATTAGCA 50.0 50.0 57.7 58.2 723-733 bp 1.0 79.6
atpF-atpH_p2 CCAGTAACCCAAGCAAAGGA TGGGCTGGTTGTAGCATTAG 50.0 50.0 57.7 57.6 725-735 bp 1.0 78.2
atpF-atpH_p3 CCAGTAACCCAAGCAAAGGA AGGCAGAGGGAAAAATACGG 50.0 50.0 57.7 57.6 792-802 bp 1.0 77.9
atpF-atpH_p4 CCAGTAACCCAAGCAAAGGA CCTGAGGCAGAGGGAAAAAT 50.0 50.0 57.7 57.5 796-806 bp 1.0 77.0
atpF-atpH_p5 CCAGTAACCCAAGCAAAGGA GCAGAGGGAAAAATACGGGG 50.0 55.0 57.7 58.6 790-800 bp 1.0 76.3