Hotspot

rps16

LSC · 56064-57232 · 1169 bp

Back to Dalbergia

Pi 0.0211
MarkerSeek score 73.40
Primer available yes
Label rps16

Feature Metadata

label_name
rps16
feature_type
gene
region
LSC
length_bp
1169
variable_sites
163
pi
0.0211
unique_haplotype_count
35
primer_available
yes
alignment_reliability
0.99
markerseek_score
73.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16_p1 ACCGGATCCAAACTAAAGGA AAGTCATCCACACCCTCCTT 45.0 50.0 56.1 58.3 1201-1253 bp 0.95 73.1
rps16_p2 ACCGGATCCAAACTAAAGGA AGTCATCCACACCCTCCTTT 45.0 50.0 56.1 58.3 1200-1252 bp 0.95 73.1
rps16_p3 ACCGGATCCAAACTAAAGGA GTCATCCACACCCTCCTTTT 45.0 50.0 56.1 57.4 1199-1251 bp 0.975 71.4
rps16_p4 ACCGGATCCAAACTAAAGGAA AAGTCATCCACACCCTCCTT 42.9 50.0 56.9 58.3 1201-1253 bp 0.95 71.3
rps16_p5 ACCGGATCCAAACTAAAGGAA AGTCATCCACACCCTCCTTT 42.9 50.0 56.9 58.3 1200-1252 bp 0.95 71.3