Hotspot

rpl32-trnL-UAG

SSC · 114310-115365 · 1056 bp

Back to Dalbergia

Pi 0.0695

MarkerSeek score 73.20

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1056
variable_sites: 328
pi: 0.0695
unique_haplotype_count: 33
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	ACAGGGAATTCAAAAAGTTTT	TCCTAAGAGCAGCGTGTCTA	28.6	50.0	52.3	57.9	302-1164 bp	1.0	52.0
rpl32-trnL-UAG_p2	ACAGGGAATTCAAAAAGTTTT	AGCAGCGTGTCTACCAATTT	28.6	45.0	52.3	57.8	299-1157 bp	1.0	52.0
rpl32-trnL-UAG_p3	ACAGGGAATTCAAAAAGTTTT	CCTAAGAGCAGCGTGTCTAC	28.6	55.0	52.3	57.8	303-1163 bp	1.0	52.0
rpl32-trnL-UAG_p4	ACAGGGAATTCAAAAAGTTTT	CTAAGAGCAGCGTGTCTACC	28.6	55.0	52.3	57.8	303-1162 bp	1.0	52.0
rpl32-trnL-UAG_p5	ACAGGGAATTCAAAAAGTTTTT	TCCTAAGAGCAGCGTGTCTA	27.3	50.0	53.2	57.9	302-1164 bp	1.0	52.0