Hotspot

ndhF-rpl32

SSC · 113422-114162 · 741 bp

Back to Dalbergia

Pi 0.0458

MarkerSeek score 80.90

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 741
variable_sites: 161
pi: 0.0458
unique_haplotype_count: 35
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGATCCATGAAGATTGATGTGT	GTGCGTTTCTTTGGAACTGC	36.4	50.0	55.5	58.5	685-797 bp	1.0	52.0
ndhF-rpl32_p2	ATGATCCATGAAGATTGATGTGT	GTGCGTTTCTTTGGAACTGC	34.8	50.0	56.0	58.5	686-798 bp	1.0	52.0
ndhF-rpl32_p3	AGGTATGATCCATGAAGATTGATGT	GTGCGTTTCTTTGGAACTGC	36.0	50.0	57.9	58.5	690-802 bp	1.0	52.0
ndhF-rpl32_p4	TGATCCATGAAGATTGATGTGT	AGTGCGTTTCTTTGGAACTG	36.4	45.0	55.5	56.9	686-798 bp	1.0	52.0
ndhF-rpl32_p5	GGTATGATCCATGAAGATTGATGTG	GTGCGTTTCTTTGGAACTGC	40.0	50.0	57.9	58.5	689-801 bp	1.0	52.0