Hotspot

accD-psaI

LSC · 59446-59932 · 487 bp

Back to Dalbergia

Pi 0.0428

MarkerSeek score 73.10

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 487
variable_sites: 102
pi: 0.0428
unique_haplotype_count: 35
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TTGATTCAATCGTACCGCGT	CCATTGCAATTGCCGGAAAT	45.0	45.0	58.0	58.0	492-697 bp	1.0	91.8
accD-psaI_p2	TGCTTACATTGCATTTGCGG	CCATTGCAATTGCCGGAAAT	45.0	45.0	58.0	58.0	596-801 bp	1.0	91.7
accD-psaI_p3	TTGATTCAATCGTACCGCGT	TTTCCAGGAAATCCCCAACG	45.0	50.0	58.0	58.1	1058-1263 bp	1.0	91.3
accD-psaI_p4	AAGGTTCACAAGCAGCTGAA	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.9	58.0	534-739 bp	1.0	91.3
accD-psaI_p5	TTGATTCAATCGTACCGCGT	CCAGGAAATCCCCAACGAAA	45.0	50.0	58.0	58.1	1055-1260 bp	0.975	90.3