Hotspot

rps16-accD

LSC · 57233-57939 · 707 bp

Back to Dalbergia

Pi 0.0345

MarkerSeek score 73.20

Primer available yes

Label rps16-accD

Feature Metadata

label_name: rps16-accD
feature_type: igs
region: LSC
length_bp: 707
variable_sites: 143
pi: 0.0345
unique_haplotype_count: 33
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-accD_p1	GGTGCTCAACCTACAGGAAC	AGAATTGAACCACCATTTTTCCA	55.0	34.8	58.2	57.1	556-850 bp	0.975	58.4
rps16-accD_p2	GGTGCTCAACCTACAGGAAC	ACAACATAGAATTGAACCACCA	55.0	36.4	58.2	55.9	563-857 bp	0.975	56.7
rps16-accD_p3	TTTCCTTGAACAGGGTGCTC	AGAATTGAACCACCATTTTTCCA	50.0	34.8	58.0	57.1	837 bp	0.025	9.8
rps16-accD_p4	TTTCCTTGAACAGGGTGCTC	ACAACATAGAATTGAACCACCA	50.0	36.4	58.0	55.9	844 bp	0.025	8.1
rps16-accD_p5	TTTCCTTGAACAGGGTGCTC	GAATTGAACCACCATTTTTCCA	50.0	36.4	58.0	55.7	836 bp	0.025	6.4