Hotspot

trnQ-UUG-rps16

LSC · 55633-56063 · 431 bp

Back to Dalbergia

Pi 0.0335

MarkerSeek score 74.20

Primer available yes

Label trnQ-UUG-rps16

Feature Metadata

label_name: trnQ-UUG-rps16
feature_type: igs
region: LSC
length_bp: 431
variable_sites: 77
pi: 0.0335
unique_haplotype_count: 34
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-rps16_p1	GAATCAAAAATGGGGCGTGG	GTCTTTCAAGTCGCACGTTG	50.0	50.0	58.0	58.0	557-608 bp	1.0	91.9
trnQ-UUG-rps16_p2	GAGGTTCGAATCCTTCCGTC	GTCTTTCAAGTCGCACGTTG	55.0	50.0	58.1	58.0	499-550 bp	1.0	91.3
trnQ-UUG-rps16_p3	GTCGAATCAAAAATGGGGCG	GTCTTTCAAGTCGCACGTTG	50.0	50.0	58.1	58.0	560-611 bp	1.0	91.2
trnQ-UUG-rps16_p4	GAATCAAAAATGGGGCGTGG	ATGTCTTTCAAGTCGCACGT	50.0	45.0	58.0	58.1	559-610 bp	1.0	90.9
trnQ-UUG-rps16_p5	GAATCAAAAATGGGGCGTGG	GCACGTTGCTTTTTACCACA	50.0	45.0	58.0	57.8	545-596 bp	1.0	90.4