Hotspot

trnR-UCU-trnG-UCC

LSC · 52319-52759 · 441 bp

Back to Dalbergia

Pi 0.0495
MarkerSeek score 79.00
Primer available yes
Label trnR-UCU-trnG-UCC

Feature Metadata

label_name
trnR-UCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
441
variable_sites
115
pi
0.0495
unique_haplotype_count
34
primer_available
yes
alignment_reliability
0.97
markerseek_score
79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-trnG-UCC_p1 TGCACATAATTTGCGTCCAA AGCCTTCCAAGCTAACGATG 40.0 50.0 56.6 58.0 523-645 bp 1.0 80.7
trnR-UCU-trnG-UCC_p2 TGCACATAATTTGCGTCCAA CCTAGCCTTCCAAGCTAACG 40.0 55.0 56.6 58.1 526-648 bp 1.0 80.5
trnR-UCU-trnG-UCC_p3 ATGCACATAATTTGCGTCCA AGCCTTCCAAGCTAACGATG 40.0 50.0 56.4 58.0 524-646 bp 1.0 78.9
trnR-UCU-trnG-UCC_p4 ATGCACATAATTTGCGTCCA CCTAGCCTTCCAAGCTAACG 40.0 55.0 56.4 58.1 527-649 bp 1.0 78.6
trnR-UCU-trnG-UCC_p5 TGCACATAATTTGCGTCCAA CCCTAGCCTTCCAAGCTAAC 40.0 55.0 56.6 57.7 527-649 bp 1.0 78.3