Hotspot

trnC-GCA-rpoB

LSC · 34403-35286 · 884 bp

Back to Dalbergia

Pi 0.0259

MarkerSeek score 73.80

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 884
variable_sites: 145
pi: 0.0259
unique_haplotype_count: 35
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	CCGGATTTGAACTGGGGAAA	GACATTCCCGCATTTTCACC	50.0	50.0	58.1	57.7	900-1029 bp	1.0	89.1
trnC-GCA-rpoB_p2	CCGGATTTGAACTGGGGAAA	GCCCCGATCAATGAACCTAC	50.0	55.0	58.1	58.4	961-1090 bp	0.975	87.1
trnC-GCA-rpoB_p3	AAAAGGATTTGCAGTCCCCC	GACATTCCCGCATTTTCACC	50.0	50.0	58.4	57.7	882-1011 bp	1.0	86.9
trnC-GCA-rpoB_p4	CCGGATTTGAACTGGGGAAA	TAAGCCCCGATCAATGAACC	50.0	50.0	58.1	57.7	964-1093 bp	0.95	86.6
trnC-GCA-rpoB_p5	CCGGATTTGAACTGGGGAAA	AGCCCCGATCAATGAACCTA	50.0	50.0	58.1	58.5	962-1091 bp	0.975	86.4