Hotspot

petN-trnC-GCA

LSC · 33339-34331 · 993 bp

Back to Dalbergia

Pi 0.025

MarkerSeek score 73.90

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: LSC
length_bp: 993
variable_sites: 163
pi: 0.025
unique_haplotype_count: 35
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	TTAAAGCAGCCCAAGCAAGA	TTTCCCCAGTTCAAATCCGG	45.0	50.0	58.0	58.1	1010-1093 bp	1.0	91.3
petN-trnC-GCA_p2	TTAAAGCAGCCCAAGCAAGA	GGGGGACTGCAAATCCTTTT	45.0	50.0	58.0	58.4	1028-1111 bp	1.0	89.1
petN-trnC-GCA_p3	GCCCAAGCAAGACTCACTAT	TTTCCCCAGTTCAAATCCGG	50.0	50.0	57.6	58.1	1002-1085 bp	1.0	88.1
petN-trnC-GCA_p4	AGCCCAAGCAAGACTCACTA	TTTCCCCAGTTCAAATCCGG	50.0	50.0	58.6	58.1	1003-1086 bp	1.0	86.2
petN-trnC-GCA_p5	GCCCAAGCAAGACTCACTAT	GGGGGACTGCAAATCCTTTT	50.0	50.0	57.6	58.4	1020-1103 bp	1.0	85.9