Hotspot

trnS-UGA-psbC

LSC · 25840-26079 · 240 bp

Back to Dalbergia

Pi 0.0405

MarkerSeek score 73.90

Primer available yes

Label trnS-UGA-psbC

Feature Metadata

label_name: trnS-UGA-psbC
feature_type: igs
region: LSC
length_bp: 240
variable_sites: 46
pi: 0.0405
unique_haplotype_count: 30
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbC_p1	CCGGTATAGTTCGCACCAAA	CCGGTTCTTTCCATGACTCC	50.0	55.0	58.0	58.3	313-329 bp	1.0	89.8
trnS-UGA-psbC_p2	AAACCGGTATAGTTCGCACC	CCGGTTCTTTCCATGACTCC	50.0	55.0	58.0	58.3	316-332 bp	1.0	89.8
trnS-UGA-psbC_p3	GAGAGATGGCTGAGTGGTTG	CCGGTTCTTTCCATGACTCC	55.0	55.0	58.0	58.3	352-368 bp	1.0	89.7
trnS-UGA-psbC_p4	GCTGAGTGGTTGATAGCTCC	CCGGTTCTTTCCATGACTCC	55.0	55.0	58.1	58.3	344-360 bp	1.0	89.5
trnS-UGA-psbC_p5	GGCTGAGTGGTTGATAGCTC	CCGGTTCTTTCCATGACTCC	55.0	55.0	58.1	58.3	345-361 bp	1.0	89.5