Hotspot

trnL-UAA-trnT-UGU

LSC · 13671-14868 · 1198 bp

Back to Dalbergia

Pi 0.0329

MarkerSeek score 82.10

Primer available yes

Label trnL-UAA-trnT-UGU

Feature Metadata

label_name: trnL-UAA-trnT-UGU
feature_type: igs
region: LSC
length_bp: 1198
variable_sites: 230
pi: 0.0329
unique_haplotype_count: 35
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnT-UGU_p1	TAGCGTCTACCAATTTCGCC	GCGATGCTCTAACCTCTGAG	50.0	55.0	58.1	57.9	784-1370 bp	1.0	90.4
trnL-UAA-trnT-UGU_p2	CGTTTCCATACCAAGGCTCA	GCGATGCTCTAACCTCTGAG	50.0	55.0	57.9	57.9	819-1405 bp	1.0	90.1
trnL-UAA-trnT-UGU_p3	ACGTTTCCATACCAAGGCTC	GCGATGCTCTAACCTCTGAG	50.0	55.0	57.9	57.9	820-1406 bp	1.0	90.1
trnL-UAA-trnT-UGU_p4	TAGCGTCTACCAATTTCGCC	GGAATCGAACCGATGACCAT	50.0	50.0	58.1	57.8	816-1402 bp	1.0	89.5
trnL-UAA-trnT-UGU_p5	CCCATTGTTAATTCCGGGGT	GCGATGCTCTAACCTCTGAG	50.0	55.0	57.8	57.9	1318-1405 bp	0.925	86.7